Text test questions 7 страница

1. How is it possible to verify the motility of bacteria which form colony?

A. Staining according to Leffler’s technique

B. Staining according to Gram’s technique

C. *By «hanging» drop technique

D. By electron microscope

E. By «lying» drop technique

1. In an autoclave at 112 °С nutrient media were sterilized. What test-microbe is it possible to utilize for sterilization control?

A. Bacillus cereus

B. Bacillus subtilis

C. Clostridium perfringens

D. Clostridium difficile

E. *Clostridium sporogenes

1. In an autoclave at 121 °С nutrient media were sterilized. What test-microbe is it possible to utilize for sterilization control?

A. Bacillus cereus

B. Bacillus subtilis

C. Clostridium perfringens

D. Clostridium difficile

E. *Bacillus steatothermophilus

1. In burner’s flame it is possible to sterilize everything, EXCEPT:

A. forceps

B. bacteriological loops

C. *tampons for material collection

D. slide glasses

E. cover slip

1. In the process of pure culture identification the most value has:

A. Morphological identification

B. Biological identification

C. Biochemical identification

D. *Serological identification

E. All answer are correct

1. In what period after finishing the disinfections is it recommended to collect the control samples?

A. not before, than in 10-15 min

B. not before, than in 15-20 min

C. not before, than in 20-30 min

D. *not before, than in 30-45 min

E. not before, than in 60-90 min

1. Indicate molecular-genetic methods that can be utilized for identification of bacteria

A. Reactions of hybridization of DNK and RNK (reaction of gene probes)

B. Polymerase chain reaction

C. Immunofluorescence test

D. *Right answers A and B

E. Right answers A, B and C

1. Is it necessary to examine the structure of colonies. What method can you offer?

A. *In the passing light at the small increase of microscope

B. In an electron microscope

C. By a "hanging" drop technique

D. By a phase-contrast microscope

E. By the immersion system of microscope

1. It is necessary to verify the hemolytic properties of bacteria. What nutrient media will you recommend?

A. simple MPA

B. simple MPB

C. Endo’s medium

D. *blood MPA

E. serum MPA

1. What nutrient media will you recommend for examenation the hemolytic properties of bacteria?

A. simple MPA

B. simple MPB

C. Endo’s medium

D. *blood MPA

E. serum MPA

1. What nutrient medium will you recommend for examenation the peptolytic properties of bacteria?

A. MPA

B. Endo’s medium

C. Ru’s medium

D. % alkaline peptone water

E. *MPB

1. It is necessary to conduct pasteurization of juice. Choose its optimum mode:

A. °С during 5-10 min

B. °С during 30 min

C. °С during 60 min

D. °С during 30 min

E. *80 °С during 5-10 min

1. It is necessary to conduct pasteurization of milk. Choose its optimum mode:

A. *70 °С during 30 min

B. °С during 60 min

C. °С during 90 min

D. °С during 30 min

E. °С during 60 min

1. It is necessary to sterilize material that contains the spore-forming bacteria by live steam. Choose the regime of sterilization:

A. single sterilization during 10 min.

B. single sterilization during 20 min.

C. fractional sterilization – three times one after the other during one day, 30 min.

D. fractional sterilization – 2 days one after the other, 30 min.

E. *fractional sterilization – 3 days one after the other, 30 min.

1. It is necessary to sterilize the dressing material. Choose optimum regime of sterilization in an autoclave:

A. °С during 20 minutes

B. °С during 20 minutes

C. °С during 40 minutes

D. *120 °С during 20 minutes

E. °С during 20 minutes

1. It is necessary to study bacterial peptolytic properties. Indicate the proper medium:

A. Meat-peptone agar

B. Sugar MPA

C. Coagulated serum

D. *Meat-peptone broth

E. gelatin

1. It is possible to sterilize in burner’s flame:

A. liquid nutrient media

B. physiological solution

C. *forceps

D. solid nutrient media

E. catheters

1. Mechanism of antibacterial effect of 37-40 % formaldehyde solution of:

A. destruction of cellular lipids.

B. *Interaction with protein amino groups and their denaturation

C. destruction of cellular endotoxin

D. destruction of cellular polysaccharides.

E. Cell dehydration

1. Peculiarities of S-colonies of bacteria:

A. *smooth, convex surface

B. gyrose surface

C. fibred consistency

D. edges are rosette-like

E. have capsules

1. Rubber and polymeric wares can be sterilized immersing them in:

A. *solution of dexon on 45 min at 18 °С

B. solution of dexon on 25 min at 18 °С

C. solution of dexon on 15 min at 18 °С

D. solution of dexon on 45 min at 56 °С

E. solution of dexon on 25 min at 56 °С

1. Serological identification is examination of bacterial species by:

A. Bacteria staining

B. *agglutinating sera

C. laboratory animals

D. biochemical signs

E. character of growth on/in nutrient media

1. Specify, to what pressure in an autoclave a temperature 100 °С corresponds:

A. *0 аtm.

B.,5 аtm.

C. аtm.

D.,5 аtm.

E. аtm.

1. Specify, to what pressures in an autoclave a temperature 112 °С corresponds:

A. аtm.

B. *0,5 аtm.

C. аtm.

D.,5 аtm.

E. аtm.

1. Specify, to what pressures in an autoclave a temperature 121 °С corresponds:

A. аtm.

B.,5 аtm.

C. *1 аtm.

D.,5 аtm.

E. аtm.

1. Specify, to what pressures in an autoclave a temperature 127 °С corresponds:

A. аtm.

B.,5 аtm.

C. аtm.

D. *1,5 аtm.

E. аtm.

1. Specify, to what pressures in an autoclave a temperature 131 °С corresponds:

A. аtm.

B.,5 аtm.

C. аtm.

D.,5 аtm.

E. *2 аtm.

1. The first stage of isolation of pure culture of anaerobes is:

A. Inoculation of material in the melted sugar agar

B. *Inoculation of material in Kitt-Tarozzi’s medium

C. Inoculation of material in a coagulated serum

D. Inoculation of material on the Zeissler’s blood-sugar agar

E. Inoculation of material on Endo’s medium

1. The second stage of isolation of pure culture of aerobes is:

A. Macroscopic examination of material and its inoculation in liquid nutrient medium

B. Macro- and microscopic verification of culture purity

C. Serological identification

D. *Macro- and microscopic study of colonies and their inoculation on slant agar

E. Biochemical identification

1. The structure of colonies can be examined by such method:

A. *In the passing light, small objective of microscope

B. In an electronic microscope

C. By a "hanging" drop technique

D. In a phase-contrast microscope

E. By the immersion system of microscope

1. Tinctorial properties of bacteria – there is:

A. character of growth

B. *character of bacterial staining

C. presence of flagella

D. antigen properties

E. all answers are correct

1. Wares from polymeric materials may be sterilized by such method:

A. mechanical

B. *chemical

C. biological

D. pasteurization

E. in a heat oven

1. Wares from rubber and polymeric materials may be sterilized by immersing them in:

A. % hydrogen peroxide, 6 hours at 18 °С

B. % hydrogen peroxide, 12 hours at 18 °С

C. % hydrogen peroxide on 1 hour at 18 °С

D. *6 % hydrogen peroxide on 6 hours at 18 °С

E. % hydrogen peroxide on 2 hour at 18 °С

1. What is tyndalization?

A. single sterilization by water bath at 98-100 °С during 30 minutes

B. *sterilization by water bath at 58-60 °С during a hour 5-6 day one after the other

C. sterilization by water bath at 98-100 °С during a hour 5-6 day one after the other

D. sterilization by water bath 58-60 °С during a hour 2-3 days one after the other

E. sterilization by water bath at 98-100 °С during a hour 5-6 days one after the other

1. What microorganisms can be used for quality control of sterilization by filtration?

A. Escherichia coli

B. Salmonella typhi

C. *Serratia mаrcescens

D. Clostridium perfringens

E. Corynebacterium xerosis

1. What antigens can be found in Enterobacteriaceae during serological identification?

A. O

B. H

C. K

D. *All answers are correct

E. All answers are wrong

1. What are cultural properties of microorganisms?

A. Features of bacterial staining

B. Features of growth of bacteria in the column of agar

C. Features of chemical composition of bacteria

D. *Features of bacterial growth on/in nutrient media

E. It is a manifestation of tinctorial signs

1. What are feature of inoculation of anaerobes by Veinberg’s technique?

A. *Dilute material from Kitt-Tarozzi’s medium in tubes with melted sugar meat-peptone agar, their cultivation then

B. Dilute material from Kitt-Tarozzi’s medium in tubes with MPB, their cultivation then

C. Dilute material from 1 % alkaline peptone water in tubes with melted sugar MPA, their cultivation then

D. Dilute material from 1 % alkaline peptone water in tubes with MPB, their cultivation then

E. Transfer material in a test tube with melted sugar MPA, its cultivate then

1. What are features of І stage of isolation of pure culture of anaerobes?

A. Macroscopic examination of tested material and its inoculation in Kitt-Tarozzi’s medium and milk

B. Microscopic examination of tested material and its inoculation in Kitt-Tarozzi’s medium and milk

C. Macroscopic and microscopic examination of tested material and its inoculation on the slant agar and in milk

D. Macroscopic and microscopic examination of tested material and its inoculation in MPB and on MPA

E. *Macroscopic and microscopic examination of tested material and its inoculation in the Kitt-Tarozzi’s medium and milk

1. What bacterial colony?

A. Medium which is inoculated with bacteria, where different microorganisms grow

B. Growth of microbes of one species on different nutrient medium

C. *Macroscopically visible growth of microbes (posterity of one bacterial cell) on a solid nutrient medium

D. Visible growth of bacteria in a liquid nutrient medium

E. Visible growth of bacteria on the slant meat-peptone agar

1. What basic principles are used for isolation of pure cultures?

A. Isolation and analysis

B. Physical and chemical

C. Biochemical and serum

D. Quantitative and qualitative

E. *Mechanical and biological

1. What biological properties are not taken into consideration for pure culture isolation?

A. Type of respiration

B. Motility

C. Spore formation

D. *Type of reproduction

E. Acid resistance

1. What can be the form of rough surfaces of colonies of R-form of bacteria?

A. *wrinkled

B. gyrose

C. warty

D. rosette-shaped

E. all answers are correct

1. What is a name of standard agglutination test for examination of bacterial antigens?

A. Coons’s test

B. Coombs’s test

C. Ouchterloni’s test

D. Manchini’s test

E. *Gruber’s test

1. What is a sign of bacterial proteolytic properties in milk?

A. Formation of diffuse turbidity

B. Formation of pellicle

C. *Dissolution of casein clot

D. Formation of sediment

E. Formation of pellicle and turbidity

1. What is a sign of proteolytic properties of bacteria in a column gelatin?

A. Formation of diffuse turbidity

B. Formation of pellicle

C. Dissolution of casein clot

D. Formation of sediment

E. *Gelatin liquefacience

1. What is a tinctorial property of microbes?

A. Features of antigen composition of bacteria

B. Features of chemical composition of bacteria

C. *Features of bacterial staining

D. Features of susceptibility to phages

E. Features of manifestation of antibiotics susceptibility

1. What is advantage of Koch’s, Drigalsky’s and streak’s techniques above the Pasteur’ method?

A. Enable to study biochemical properties of bacteria

B. Enable to study antigen properties of bacteria

C. Enable to obtain a pure culture

D. *Enable to obtain the isolated colonies

E. Right answer is not present

1. What is an essence of isolation of anaerobes pure cultures according to Zeissler’s technique?

A. Material from Kitt-Tarozzi’s medium is inoculated consistently in test tubes with a sugar-blood agar by bacteriological loop, then they are placed in the jar and incubator

B. Material from the Kitt-Tarozzi’s medium is inoculated consistently on MPA in by a spatula, then they are placed in the jar and in a thermostat

C. Material from Kitt-Tarozzi’s medium is inoculated consistently on serum agar by a spatula, then it is placed in the jar and in a thermostat

D. *Material from Kitt-Tarozzi’s medium is inoculated consistently in the Petry’s plates with sugar-blood agar by a spatula, then they are placed in the jar and in the thermostat

E. Material from Kitt-Tarozzi’s medium is inoculated consistently in test tubes with serum broth by a loop, then they are placed in the jar and in a thermostat

1. What is an essence of Koch’s technique for isolation of pure cultures?

A. *Use of melted nutrient media (gelatin) for obtaining the isolated colonies

B. Use of liquid nutrient media for obtaining the isolated colonies

C. Use of blood agar for examination of hemolysines types

D. Use of solid nutrient media for examination of antibiotics susceptibility

E. Use liquid nutrient media for examination of antibiotics susceptibility

1. What is an essence of Pasteur’s technique for isolation of pure cultures?

A. Dilution of tested material in gelatin

B. *Dilution of tested material in a liquid nutrient medium

C. Dilution of tested material in MPA

D. Dilution of tested material in the AGV nutrient medium

E. Dilution of tested material in a solid nutrient medium

1. What is an essence of ІV stage of isolation of pure culture of anaerobic bacteria?

A. Inoculation of colonies on the proper nutrient medium

B. Examination of tinctorial properties of bacteria

C. *Checking the culture purity and its identification

D. Examination of serologic features of bacteria which form colonies by precipitation test

E. Right answer is absent

1. What is bacterial colony?

A. Medium which is inoculated with bacteria, where different microorganisms grow

B. Growth of microbes of one species on different nutrient medium

C. *Macroscopically visible growth of microbes (posterity of one bacterial cell) on a solid nutrient medium

D. Visible growth of bacteria in a liquid nutrient medium

E. Visible growth of bacteria on the slant meat-peptone agar

1. What is biochemical identification of bacteria?

A. Verification of bacteria according to their ability to produce dehydrogenases

B. *Verification of bacteria according to their biochemical properties

C. Verification of bacteria according to their ability to produce hemolysines

D. Verification of bacteria according to their tinctorial properties

E. Verification of bacteria according to their reduce properties

1. What is biological identification of bacteria?

A. *Verification of bacteria according to changes which they cause in the organism of laboratory animals

B. Verification of bacteria according to their tinctorial properties

C. Verification of bacteria according to their biochemical properties

D. Verification of bacteria according to their antigen properties

E. Verification of bacteria according to their cultural properties

1. What is cultural identification of bacteria?

A. Verification of bacteria according to their tinctorial properties

B. Verification of bacteria according to their ability to form S- and R-forms of colonies

C. Verification of bacteria according to their capacity to form slime layer

D. Verification of bacteria according to their capacity to produce hydrogen sulfide

E. *Verification of bacteria according to their features of growth on/in nutrient media

1. What is disinfection?

A. *Complex of measures for complete, partial or selective elimination of potentially pathogenic for human causative agents on the different objects of medium for warning transmission of causative agents from the source of infection to the receptive organism

B. Complex measures for complete, partial or selective elimination of potential pathogens for human in the air for warning transmission of causative agents from the source of infection to the receptive organism

C. Complex of measures for complete, partial or selective elimination of potential pathogens for human in his body for warning transmission of causative agents from the source of infection to the receptive organism

D. Complex of measures which prevent spread of potential pathogens from human body in an medium

E. Complex of measures for complete, partial or selective elimination of potential pathogens for human in the water for warning transmission of causative agents from the source of infection to the receptive organism

1. What is essence of ІІІ stage of isolation of aerobes?

A. verification of culture purity

B. inoculation of Giss’ media, slide agglutination

C. examination of the antibiotics susceptibility

D. Phage typing

E. *all answers are correct

1. What is it necessary to do during the second stage of isolation of pure culture of aerobes?

A. Study the features of colonies

B. Make a smear from colonies

C. Inoculation onto slant agar

D. *All answers are correct

E. All answers are not correct

1. What is morphological identification?

A. Verification of bacteria according to their ability to form a capsule

B. Verification of bacteria according to their ability to form flagella

C. *Verification of bacteria according to their morphological signs

D. Verification of bacteria according to their capacity to spore formation

E. Verification of bacteria according to their ability to transform into L-forms

1. What is serologic identification of bacteria?

A. Verification of bacteria according to their capacity to form of lipopolysaccharide

B. Verification of bacteria according to their ability to producе bacteriocines

C. *Verification of bacteria according to their antigen properties

D. Verification of bacteria according to their capacity to produce antibodies

E. Right answer is not present

1. What does means serological identification of the pure culture?

A. Examination of bacterial species according to biochemical properties

B. Examination of bacterial species according to ability to utilize a coagulated serum

C. *Examination of bacterial species according to their antigen structure

D. Examination of bacterial species according to ability to reduce some dyes

E. Examination of bacterial species according to gene composition of bacterial nucleoid

1. What is strain?

A. *Microbes of the only species isolated from different sources, or from the same source, but at different times

B. Microbes of different species isolated from different sources

C. Microbes of different species isolated from the only source

D. Microbes of different species isolated in different times

E. Microbes of different species isolated from different sources, or from the same source, but in different times

1. What is the essence of ІІІ stage of isolation of pure cultures of aerobes?

A. Examination the isolated colonies

B. Examination bacterial growth in the column of agar

C. Bacterial phage typing

D. *Identifications of the unknown bacteria

E. Examination of ability for bacteriocine production

1. What is the main demand for any nutrient medium for the primary selection of microorganisms?

A. To be solid

B. To be liquid

C. *To be sterile

D. To contain vitamins

E. To contain growth factors

1. What is the main idea of ІІІ stage of isolation of pure culture of anaerobic bacteria?

A. Examination of features of pure culture and its identification

B. *Examination of features of colonies, preparing the smear from them, inoculation of colonies into Kitt-Tarozzi’s medium for obtaining the pure culture

C. Examination of features of colonies, preparing the smear from them, inoculation of colonies on slant MPA for obtaining the pure culture

D. Examination of features of colonies, preparing the smear from them, inoculation of colonies in MPB for obtaining the pure culture

E. Examination of features of colonies, inoculation of colonies in Kitt-Tarozzi’s medium for obtaining the pure culture

1. What laboratory animals are most often utilized for biological identification?

A. Guinea pigs

B. White mice

C. White rats

D. *All answers are correct

E. Right answer is not present

1. What laboratory animals are very susceptible to Mycobacterium tuberculosis?

A. White rats

B. *Guinean pigs

C. Rabbits

D. Syrian hamsters

E. White mice

1. What laboratory animals are very susceptible to Streptococcus pneumoniae?

A. White rats

B. Guinean pigs

C. Rabbits

D. Syrian hamsters

E. *White mice

1. What main idea of the first stage of isolation of pure cultures of aerobes?

A. To study tested material

B. To choose nutrient medium for inoculation

C. *To obtain the isolated colonies

D. To study the morphological features of possible causative agent

E. To study the antigen features of causative agent

1. What methods of isolation of pure cultures, based on mechanical principle do you know?

A. Leffler’s method

B. Grey’s method

C. *Streak’s method

D. Buchin’s method

E. Nemchinov’s method

1. What modern test-systems are utilized for identification of bacteria?

A. “Roche”

B. “API”

C. “Enterotest”

D. *All answers are correct

E. All answers are wrong

1. What nutrient medium is it possible to use for examination of bacterial proteolytic properties?

A. Endo’s medium

B. Olkenitsky’s medium

C. MPB

D. *Milk

E. Levenshtein-Yensen’s medium

1. What properties of bacteria are utilized for identification of anaerobic bacteria?

A. Morphological

B. Signs of growth in/on nutrient media

C. Biochemical

D. Toxigenic

E. *All answers are correct

1. What properties of colonies do we study first?

A. Shape, color

B. Character of edges

C. Character of surface of colonies

D. A right answer is not present

E. *All answers are correct

1. What substances can repress the action of inhibitors of bacterial growth and toxin formation?

A. some amino acids

B. tweens

C. active carbon

D. *all answers are correct

E. all answers are wrong

1. Why do we use Vinyal-Veyon’s tubes?

A. *For cultivation of anaerobes according to Veinberg’s technique

B. For cultivation of anaerobes according to Zeissler’s technique

C. For cultivation of aerobes according to Ziehl–Neelsen’s technique

D. For cultivation of aerobes according to Leffler’s technique

E. In bacteriology they are not used

1. Why is it necessary to identify the pure cultures of bacteria?

A. for prescribing the treatment

B. *for revealing the etiological factor illness

C. for prophylaxis

D. for examination of phages susceptibility

E. for examination of antibiotics susceptibility

1. With the purpose of increase of boiling temperature and removal of water hard during sterilization by boiling it is necessary to add:

A. *1 % sodium bicarbonate

B. % potassium permanganate

C. % magnesium sulfate

D. % ether

E. % alcohol

1. A nurse has to sterilize surgical instruments for an operation. What most effective method can you offer?

A. fractional sterilization

B. sterilization by pressed steam

C. boiling

D. *by dry heat

E. ionizing radiation

1. After finishing the experience in students’ laboratory it’s necessary to disinfect the workplace. What chemicals can be used?

A. ether

B. hydrochloric acid

C. formalin

D. chloroform

E. *Chloraminum

1. At what advantage of Koch’s method in comparing Pasteur’s one for isolation of pure cultures?

A. Enables to examine antibiotics susceptibility

B. Enables to examine biochemical properties of microbes

C. *Enables to obtain the isolated colonies

D. Enables to examine antigen properties of microbes

E. Has no substantial advantage

1. For sterilization of glassware utilize a heat oven is used. Choose the best regime of sterilization:

A. C, 15-30 minutes

B. 1C, 120 minutes

C. *180 1, 45-60

D. 1, 45-60 minutes

E. 1, 15-30

1. From patient with gas anaerobic infection it is necessary to select the culture of causative agents. What medium can be chosen?

A. Leffler’s medium

B. *Kitt-Tarozzi’s medium

C. Petrov’s medium

D. Makkoy-Chepin’s medium

E. Petranyani’s medium

1. From patient’s blood the culture Salmonella typhi was isolated. What cultural properties characterize this microbe?

A. *Formation of colorless or pinky colonies on Endo’s and Ploskirev’s nutrient media

B. Formation of red colonies with metallic hue on Endo’s medium

C. Formation of colorless colonies on Bismuth-sulfite agar

D. Formation of haemolysis on a blood agar

E. Formation of tender pellicle on alkaline peptone water

1. In a medium with gelatin it is possible to verify the proteolytic properties of bacteria. What signs will show the positive results?

A. *liquefacience of gelatin column

B. compression of gelatin column

C. discoloration of gelatin column

D. appearance of dark precipitate in gelatin column

E. brightening of gelatin column.

1. In heat oven at 160 C Petry’s palates were sterilized. What test-microbe is it possible to use for sterilization control?

A. Bacillus cereus

B. *Bacillus subtilis

C. Clostridium perfringens

D. Clostridium difficile

E. Proteus vulgaris

1. In Olkenitsky’s medium microorganism which products an urea was inoculated. What changes here will be observed in a medium?

A. *all will become red

B. appearance of red precipitate in the column of agar

C. appearance of greenish precipitate in the column of agar

D. to fix the urease production in this medium is impossible

E. appearance of brightly-violet precipitate in the column of agar

1. It is necessary to check the quality of antibacterial filters. What microorganism is it possible to choose as a test-object?

A. *Pseudomonas aeruginosa

B. Salmonella schottmuelleri

C. Staphylococcus aureus

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