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Principal methods of study of morphology and function of cells.

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Theme 1

Levels organization of Living things.

Principal methods of study of morphology and function of cells.

1. What two structures on the microscope will you use to focus on your specimen? __________________________________________
2. Why should you never use the coarse adjustment knob on high power? ___________________________________________________________________________________________
3. What will happen if you use direct sunlight to observe your specimen?
______________________________________________________________________________________
4. Our microscopes have three objectives. What are their powers?________________________
5. What is the magnification of the ocular lens? ____________________
6. What is the shortest objective called? ________________________________________
7. How do you switch objectives?____________________________________________________________
8. Which structure controls how much light passes through the specimen?______________________
9. You should carry the microscope by placing your palm on the _____________________ and gripping the ___________________ with your other hand.
10. How can you prevent your slide from slipping on the stage?________________________________

11. DIRECTIONS: Before using the microscopes in the lab, the Microscope Tutorial Program (installed on the desktop in the computer lab under Biology Tutorials) and the following quiz must be completed. While running the tutorial, match the following. Words may be used more than once or not at all.


A. Limit of Resolution

B. Numerical Aperture

C. Light Microscopy

D. Scanning Electron Microscopy

E. Total Magnification

F. Working Distance

G. Depth of Field

H. Field of View

I. Field Size

J. Electron Gun

K. Condenser Lens

L. Electromagnetic Lenses

M. Ion Pump

N. Specimen Stage

O. EDS Detector

P. Sputter Coater

Q. Secondary Electron Detector

R. Photomicrograph

S. Micrometer

T. Objective Lens


______ 1. Relies on the bending, or refraction of light rays.

______ 2. The visual circle of light.

______ 3. The amount of enlargement of the specimen.

______ 4. One millionth of a meter.

______ 5. Deposits a thin layer of metal over the specimen.

______ 6. Produces the primary electron beam.

______ 7. Influences intensity or brightness of light beam.

______ 8. Use magnetic fields to control primary electron beams.

______ 9. Detects x-rays emitted from an SEM specimen.

______10. The space between the objective lens and the specimen.

______11. The smallest detail which we may clearly see.

______12. A measure of the quality of the lens.

______13. A platform upon which the specimen rests.

______14. A picture of a magnified specimen or feature.

______15. The thickness of specimen that is in focus.



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