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The main types of cells. Darkfield. Scanning and fluorescence microscopy. Study of the main features of plant and animal cells. Terrestrial and aquatic plants. The simplest
Aim
Mastering the basic methods and approaches biological microscopy
Task
Study of the main features of plant and animal cells. Terrestrial and aquatic plants. The simplest
Theoretical information
Microscopy is wide used to study the morphology and ultrastructure of the cells to determine type of cells, genus, species, and to determine the properties, functions and roles of individual cells in tissues, organisms, natural processes using the following methods:
microscopic, in which a specially made preparations (smear, crushed drop, etc..) under a microscope studying the shape, size, structure, relation to staining cells, cell motility;
samples, which involves seeding cells in culture media, isolation of individual species from each other and explore the nature of the growth of pure cultures on nutrient media;
chemistry, which allows to determine the selected cells belonging to a particular type, type, type on its biochemical (enzymatic) activity of cells or to clarify the role they play in the tissues, organisms in the circulation of substances in nature;
sero-based identification (identification) of selected bacteria and determining their species and typical accessories for their antigenic structure by antibodies contained in the antiserum;
Biology in microbiological laboratories, allowing separate pathogenic for humans, animals and plants from saprophytic microbes. It is produced by infection of experimental animals or plants microbe, dedicated to pure culture
| field of view diameter(about), µm | ||||
| Objective piece magnification | Х20 | Х25 | Х40 | Х90 |
| field of view diameter, µm |
Equipment, materials and methods.
Microscope objective lenses, glasses with hole, glass rods, hinges, cover lenses.
Bacteria, plants
The order of performance and recommendations
- Obtain samples of living cells and tissues.
- Prepare for microscopy native (unpainted preparates) types "hanging drop" and "crushed drop."
- Prepare smears and other preparations for painting.
- Fix samples chemically and thermally
- Carry out staining technique.
- Make a microscopy observations of obtained preparations. Fix the image on all available degrees of magnification, from lower to most.
- Compare view of cells fixed and painted and native.
- Set clear image of changing the position of condenser.
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