C2005/F2401 '06 -- Recitation Problems #11 -- Hints

1. A-1. What is the definition of an intron? Are all nontranscribed regions of the DNA introns?
A-2. If you had a choice, which strand would you show??

B-1. How long is the peptide, and how long are the exons? Do all 3 exons have to contain sequences that code for the peptide? Could translation start in the second exon?
B-2. What is the relationship between the position of the polyA addition site and the end of transcription?
B-3. See B-1. Could translation end in the second exon?

C-1. How many pieces will you get from the region shown? How many of those pieces will hybridize to cDNA? When you consider the lengths of the pieces, remember that the DNA shown is part of a longer DNA molecule.
C-2. How could one band be darker than another? How could it trap more probe?

D-1. Do you want to cut inside the DNA you are trying to clone?
D-2. What enzyme will cut up the genomic DNA but NOT the cDNA? Can you use it?

2. For the karyotype: What does each type of chromosome look like at metaphase? How many chromatids, and how are they connected? How do you arrange the chromosomes in a karyotype?

A. Check out the definitions of C and N.

B-1. How many chromatids/chromosome at metaphase? How are they attached to the spindle?
B-2. How many chromatids/chromosome at anaphase? How are they attached to the spindle?

C. Has the DNA replicated in G-2? How many DNA molecules per chromosome and/or chromatid?

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