215) Temperature which is necessary for a trypsinization method, C?
А) 37
B) 10
C) 30
D) 20
E) 0
216) Most effective for increase growth upside to use:
А) beads
B) cover
C) tubule
D) minute vessel
E) glass band
217) Ions Са 2+ necessary for a medium Igla for:
А) affixion of cells to the surface of the glass
B) stain medium
C) trypsinization of cells
D) inhibition of synthesis
E) keeping up pH
218) Ions К+ and Na, present in nutriculture medium Igla for:
А) affixion of cells to the surface of the glass
B) stain medium
C) trypsinization of cells
D) inhibition of synthesis
E) keeping up pH
219) Ions К+ and Na, present in nutriculture medium Igla carry of:
А) Osmotic balance cells
B) promote keeping up pH
C) metastasis of cells
D) suppression of bacterial infection
E) inhibition of synthesis
220) Phosphates in nutriculture medium Igla provide cells by ions:
А) phosphate
B) nitrate
C) bicarbonate Na
D) calcium
E) natrium
221) Antibiotic produce consisting of nutriculture medium Igla for:
А) Suppression of bacterial infection
B) increase, alimentation
C) trypsinization of cells
D) affixion of cells to the surface of the glass
E) for keeping up pH
222) Major components universe of Igla is:
А) amino acids, vitamins, serosity
B) amino acid trypsinized cells
C) vitamins, NCL, trypsinized cells
D) serosity, vitamins, any acid
E) serosity, disaggregate tissue
223) In the presence of strong cidification universe of Igla is:
А) It changes color
B) it doesn’t change color
C) her eat the microorganisms
D) appear a new cells
E) appear a mutant cells
224) To provide a cells metabolism, an attachment and a spreading of cells on glass surfaces, and also growing cells with proteins, amino acids, vitamins etc. in the medium of Igla is presence is necessary:
А) serosity
B) antibodies
C) fibrinogen
D) callus
E) dissolver
225) Cybrids are got by the fusion of:
А) nuclear-free cells with healthy cells
B) hybrid cell
C) two nuclear-free cells
D) karyoplast and cytoplast
E) nuclear-free cells with cytoplast
226) If to fuse acariotic cell with intact cell we can get cytoplasmic:
А) cybrid
B) callus
C) protoplast
D) tissue
E) karyoplast
227) Which substances are used for enucleasion:
А) cytochalasin
B) chloranfenk(HAF)
C) bromine Br
D) GGFRT
E) serosity calf
228) What is used for enucleasion’s speed increasing:
А) centrifugation
B) extraction
C) floatation
D) absorption
E) adsorption
229) Fused nuclear erythrocytes with enucleased cells can exist:
А) up to 3 days
B) up to 7 days
C) up to 3 days
D) up to 7 years
E) up to 10 years
230) For cells growing in plasma bunch plasma and nutritional liquid of following consistence are used
А) serosity blood of calf –20%, extract of embryonic tissue –10%, medium of Erla-70%
B) serosity blood of calf –40%, extract of embryonic tissue –50%, среда Эрла-10%
C) serosity blood of calf –50%, среда Эрла-50%
D) extract of embryonic tissue -10%, среда Эрла - 90%
E) serosity blood of calf –70%, extract of embryonic tissue -30%
231) Cells, got from animal and man and supported in culture:
А) primary
B) single
C) secondary
D) tertiary
E) nonsingle
232)For checking of capability to joint growth of plant cell and cyanobacterium in suspension cultures Murasige and Skuga mediums were chosen, which are used for cultures of plant cells, in which concentration of all components were decreased:
А) in 2-4 times
B) in 5times
C) in 10 times
D) do-not-reduce
E) in 15 times
233) The class of serum proteins, which have certain qualities of antisubstances, and by structure consist of heavy and easy chains, related by ion power and disulfide bridges:
А) immunoglobulins
B) isoenzyme
C) insecticide
D) inhibitor
E) inokulum
234) The growth in long time segments culture, isolated from plant knot of different origin and set free from pathogen, induced knot development:
А) cultivation of tumor tissues
B) cultivation of apart cells
C) cultivation callus tissue
D) cultivation explant
E) cultivating
235) When one cell is landed on nutritional medium, it doesn’t divide, because of the lack of evolved conditioning factor for induction division. Consequently, it is necessary to increase factor concentration in nutritional medium with use of:
А) method nourish on “layer”
B) method hanging “drops”
C) method “hybridization”
D) rule of “gimlet”
E) esthetic associations
236) The most important factor, influenced to clonal micro-propagation of plants:
А) nutrient solution
B) temperature
C) illumination
D) humidity
E) elasticity
237) The most important factor, influenced to clonal micro-propagation of plants:
А) condition of explant
B) humidity
C) temperature
D) illumination
E) airing
238) Physical factors are important for improvement of clonal micro-propagation, for example optimal temperature of cultivation for most of plants will be close to:
А) 25 0С
B) 35 0С
C) 40 0С
D) 60 0С
E) 650С
239) Optimal intensity of lightning in clonal micro-propagation for most of plants is:
А) 1000-3000 lk during 14-16 h/day
B) 1500-5000 lk during 14-16 h/day
C) 4000 lk for 14-16 h/day
D) 4000-6000 lk during 14-16 h/day
E) 5000-7000 lk during 14-16 h/day
240) Relative humidity in chambers, where test-tube plants are, supported on level of:
А) 65-75%
B) 20-25%
C) 30-50%
D) 80-85%
E) 10-15%
241) Parasexual hybridization is executed by:
А) as interflowing protoplast
B) in a solution of acids
C) in a solution of alkalis
D) as interflowing of sexual cells
E) as interflowing of thrombocyte
242) The implication of monoclonal antibody technology:
А) as interflowing of normal cells to the transformed tumoral cells
B) reception of nuclear-free cells
C) cultivation of cells on "beads"
D) as interflowing of naive cells with a nuclea-free cell
E) cultivation of cells in a clot of plasma
243) The using of objects, fully keeping genetic stable on all levels of process, from explants to plants in ground, obligatory condition:
А) clonal microduplication
B) cultivation of cells on "beads"
C) cultivation of cells in a clot of plasma
D) as interflowing of naive cells with a nuclea-free cell
E) there is no right answer
244) The growth of cultures of plant cells in biogenerator are lead:
А) In 2 modes
B) In 1 modes
C) In 4 modes
D) In 3 modes
E) In 5 modes
245) For getting protoplasts, suspension cells are worked out by ferments solution, procedure of wall destruction is lead:
А) 30-60 minutes, at t 4-8˚C
B) 10-15minutes, at t 4-8˚C
C) 2 hours, at t 4-8˚C
D) 180 minutes, at t 4-8˚C
E) 80minutes, at t 4-8˚C
246) Protoplasts are got under action of:
А) enzyme
B) electrical current
C) congelation
D) electromagnetic field
E) mechanical shock
247) For getting of protoplasts process of cells’ walls destruction is lead, which causes condition of “shock” of cells, which has influence to:
А) Increase of number vacuole in cytoplasm
B) Increase of speed of a metabolism
C) In cells the genetic information is lost
D) Begins parasexual hybridizations
E) Cells perish
248) Coumarine is entered into medium consistence for protoplasts fusion:
А) Prevents regeneration of a cellular wall
B) is a harmful impurity
C) causes enucleation
D) increases growth rate
E) increases number of nuclear fissions
249) In protoplasts fusion in medium consistence coumarine is entered in concentration of:
А) 200-250 mg/l
B) 100-150 mg/l
C) 300-350 mg/l
D) 400-450 mg/l
E) 500-550 mg/l
250) For getting of cultivated callus cell explants are put in imitiative nutritional in vitro:
А) in Petri dish
B) electrolysis cell
C) in autoclave
D) in adapter
E) in centrifuge
251) Primary callus, arisen on explants in 4-6 weeks, transfer on a fresh nutrient medium. The size transplant at fermentation on agarized to environment surge within the limits of:
А) 60-100 mg on 30-40 ml of a nutrient medium
B) 40-50 mg on 30-40 ml of a nutrient medium
C) 120-150 mg on 30-40 ml of a nutrient medium
D) 20-40 mg on 30-40 ml of a nutrient medium
E) 30-60 mg on 30-40 ml of a nutrient medium
252) The effective inducing agent for merge protoplasts is PAG, its satisfactory molecular weight at the rate of 3 drops on 1 drop of suspension of cells how many makes:
А) 1540-6000
B) 1000-1200
C) 200-800
D) 7500-9000
E) 80-200
253) For solution ПЭГ for interflowing protoplasts prepare, 1 g ПЭГ to 2 ml of a solution of the glucose of 0,1 m, containing 10,5 ммколь/л and CaCL2 and 0,7 ммколь/л КН2 РО4, how in this case his molecular weight:
А) 1540
B) 1005
C)) 1250
D) 1340
E) 1450
254) For reception of products of interflowing mesophyll protoplast the most effective inducing substance is:
А) Ca
B) Mg
C) К
D) Na
E) Р
255) The most circulated method at reception by a hybrid is:
А)GAT
B) PAG
C) PAV
D) HAV
E) DNA
256) The basic-type environment used at reception by a hybrid:
А) RPMI
B) Nagata and Takabe
C) Whitey
D) Gamborg and Evelina
E) Murasik-Skuga
257) What natural medium habitation of tuberculose bacteria Rhizobium sp:1, air
2. Ground; 3. Water; 4. Internal cavities of warm-blooded organisms; 5. The Mouth of the blood person; 6. Dairy products:
А) 2
B) 3,5
C) 5,6
D) 1,4,5
E) 3,4
258) At a high relationship of hormones in the environment at progress callus the formation is under way:
А) Burgeon
B) Callus
C) Roots
D) Stalks
E) Petals
259) At a necessary relationship of hormones in the environment at progress callus the formation is under way:
А) Roots
B) Stalks
C) Burgeon
D) Callus
E) Petals
260) What blood corpuscles contain enzyme – hemoglobin:
А) red blood corpuscle
B) monocyte
C) lymphocyte
D) basophils
E) nestrophylis
261) The minimal time necessary for sterilization of environment in the way of autocloning, depends on volume of environment in retort. For example, for Shenk- Hildebrandt environment for volumes of 250-500 ml spend autocloning:
А) In current of 25 mines at 120ºС
B) In current of 15 mines at 120ºС
C) In current of 35 mines at 120ºС
D) In current of 45 mines at 120ºС
E) In current of 60 mines at 120ºС
262) For cells of the higher plant, size of pH of environment usually before avtoklavium lead up up to:
А) 5,5-6,0
B) 7,5-7,7
C) 8,0-10,0
D) 10,5-12,0
E) 13,5-14
263) At immunization by a complex hapteneprotide it is formed up to:
А) 80 000 various antibodies
B) 10 000 various antibodies
C) 1 000 various antibodies
D) 50 000 various antibodies
E) 100 000 various antibodies
264) To protect cytoplasm, to provide constant endocellular osmotic pressure, to adjust delivery of nutrients in a cell fulfil a function:
А) cell membrane
B) meristematic tissue
C) fibrous tissue
D) bindweb
E) biosynthesis
265) At combined fermentation vegetative cells and cyanobacterium, stimulating influence cyanobacterium on increase of vegetative cells of bacteria is revealed:
А) tobacco, ginseng
B) leguminous plant
C) potatoes, rape
D) tomato, potatoes
E) corns, a garden radish
266) Primary callus, appear on explant, depending on paces of increase it is transferred on a fresh nutritional medium:
А) 4-6 weeks
B) 12 weeks
C) 15 weeks
D) 24 weeks
E) 30 weeks
267) The size of transplant at fermentation agarized to environment on 30-40 ml surge from:
А) 60-100 mg of weight of tissue
B) 250-300 mg of weight of tissue
C) 500 mg of weight of tissue
D) 10-20 mg of weight of tissue
E) 30-50 mg of weight of tissue
268) For initiation suspended cultures it is necessary 2-3 г fresh weight for a callus tissue for a liquid nutrient medium:
А) 60-100 ml of medium
B) 250-300ml of medium
C) 500 ml of medium
D) 10-20ml of medium
E) 30-50ml of medium
269) Before the first subculturing and in the subsequent subculturing, before purchase by suspension of desirable characteristics, callus suspension:
А) filter
B) centrifuge
C) sediment
D) distill
E) sterilize
270) To provide and stimulate process of merge protoplasts various toxins, for the first time has shown:
А) Konking
B) Koh
C) Poster
D) Murasig-Skuga
E) Robbins
271)Kokking for the first time has carried out process of merge protoplasts, the roots of sprouts of an oats received from tips and a corn and used as helical coil:
А) 0,25 М NaNO3
B) PAG
C) PAV
D) NaOH
E) ions Ca2+
272) At carrying out of hybridization nuclear-free L-cells of the mouse, for the first time L-cells stable to HAF, the second – BU. What cells survive on the selective environment containing HAF and BU after their merge:
А) cybrid, with substantial to HAF
B) not symphysic cells
C) cell substantial of HAF
D) cell substantial of BU
E) hybrid substantial of HAF and BU
273) For the purpose of appear burgeon from callus to environment we need adjust
А) vitamins
B) auxin and kinetin
C) hormones
D) macroelement
E) carbohydrate
274) Increase of number of cells by mitosis, leading growth of a fabric:
А) proliferation
B) regenerate
C) cultivating
D) interflowing
E) oxidation
275) Introduction of microorganisms in isolated protoplasts plants spend influence on them:
А) Inducing factors
B) Biopower factors
C) Genetic factors
D) Chemical factors
E) Physical factors
276) At preparation of firm nutrient mediums for superficial cultivation callus tissue use:
А) An agar-agar
B) Water
C) Spirit
D) Paper
E) Protoplast
277) Cultivation in long culture of the segments isolated from vegetative tumours of a different origin and released from pathogen, induced progress of a tumour:
А) Culture of tumoral tissue
B) Culture of separate cells
C) Culture callus tissue
D) Culture explant
E) Cultivation
278) The bacterial cell accept a part of a genetic material of a cell of the donor:
А) the recipient
B) producer
C) ribosome
D) aerobe
E) anaerobe
279) To receive protoplasts: it is necessary to remove a cellular wall;
А) To place a cell in an acid
B) To remove a cellular membrane
C) To place a cell in a solution of phosphate
D) Mechanically to destroy a cell
E)
280) Artificial associations of cells of the maximum plants and m.о. Can be used for the decision of following practical problems:
А) Increase of efficiency of a vegetative cell at cultivation in culture
B) Possibility to receive new strain m.о. with set catabolic properties
C) Such associations now yet have no practical significance
D) Increase of efficiency of microbic cells at them культивировании in a bioreactor
E) Such associations now; are not created, but are perspective in the future
281) To reduce fragile protoplasts from euzymatic mixture, it is possible to use a method:
А) Flotations
B) Rotation
C) Maceration
D) Filtrations
E) Conjugation
282) For challenges of capacity for the compound height of vegetable cages and cyanobacterias in suspension cultures it is possible to apply an environment:
А) Murasige-Skuga
B) Paster
C) meat infusion agar
D) Skuga-Nerevstovsk
E) starvation agar with 1 % NaCl
283) Work with the culture of tissue of plants is impossible without:
А) Rocking chairs for suspended cultures
B) Availability of a standard set of acids and alkalis
C) Availability of a standard set of amino acids
D) Introductions of progressive technologies
E) Studying of influence pH and t on growth rate
284) For regulation of height of plants it is possible to use:
А) auxin and cytokinin
B) auxin and cytisin
C) auxin and cytoxin
D) aukinin and cytoxin
E) solutions and permanganate
285) What equipment is needed for experiments on confluence of protoplasts mesophyll and albinotic plants:
А) Chamber Murasige-Skuga
B) Laminar box
C) Microscope
D) Neutral a sieve in diameter 64 microns
E) Chamber Fukla-Rezentalja
286) If at the synthesis of products of secondary metabolism cellular cultures is sharp suppression of synthesis, one of the reasons of it can be:
А) Syntheticauxin 2,4D
B) Decrease in temperature on 1С
C) Rise in temperature on 1С
D) Synthetic cytokinin 2,4c
E) Synthetic cytokinin 2,4D
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